Fluorescence Recovery after Photobleaching in Ultrathin Polymer Films
نویسندگان
چکیده
منابع مشابه
Novel insights in membrane biology utilizing fluorescence recovery after photobleaching.
25-NBD-cholesterol 25-[ N -[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)methyl]amino]27-norcholesterol 5-HT 1A receptor 5-Hydroxytryptamine-1A receptor 5-HT 1A R-EYFP 5-Hydroxytryptamine-1A receptor tagged to enhanced yellow fl uorescent protein DiIC 18 (3) 1,1′-Dioctadecyl-3,3,3′,3′,-tetramethylindocarbocyanine perchlorate EYFP Enhanced yellow fl uorescent protein FAST DiI 1,1′-Dilinoleyl-3,3,3′,3′,-te...
متن کاملPhotobleaching of Fluorescent Dyes in Polymer Films
Recently, optical data storage has been achieved via photobleaching of a fluorescent dye in co-extruded, multilayer, polymer films. Photobleaching, the decrease in fluorescence by exposure to light, can be caused by a number of different processes, including light-induced chemical reactions and thermal degradation. Although data has successfully been written using this method, the mechanisms co...
متن کاملProtein Synthesis Rate Assessment by Fluorescence Recovery after Photobleaching (FRAP).
Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically different translation rates, in live Caenorhabditis elegans animals.
متن کاملFluorescence recovery after photobleaching: the case of anomalous diffusion.
The method of FRAP (fluorescence recovery after photobleaching), which has been broadly used to measure lateral mobility of fluorescent-labeled molecules in cell membranes, is formulated here in terms of continuous time random walks (CTRWs), which offer both analytical expressions and a scheme for numerical simulations. We propose an approach based on the CTRW and the corresponding fractional d...
متن کاملFluorescence recovery after photobleaching: application to nuclear proteins.
Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological research. The method was developed in the 1970s, when its biological application mainly focused on the mobility of fluorescently labelled constituents of the cell membrane. The development of confocal scanning microscopy in the 1980s facilitated accur...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Macromolecular Chemistry and Physics
سال: 2018
ISSN: 1022-1352
DOI: 10.1002/macp.201700543